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The effect of epitope density and antibody affinity on the ELISA as analysed by monoclonal antibodies.

Journal of immunological methods (1984-08-03)
A M Lew
ABSTRACT

The antibody titre and the optical density values in an ELISA are influenced by the epitope density of the antigen and the affinity of the antibody tested. This has major implications in the interpretation of ELISA results.

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Sigma-Aldrich
IgG1, Lambda from human myeloma plasma, purified immunoglobulin, >95% (SDS-PAGE)