- Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits.
Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits.
Science (New York, N.Y.) (1994-11-11)
T Miyazaki, A Kawahara, H Fujii, Y Nakagawa, Y Minami, Z J Liu, I Oishi, O Silvennoinen, B A Witthuhn, J N Ihle
PMID7973659
ABSTRACT
The interleukin-2 receptor (IL-2R) consists of three subunits: the IL-2R alpha, IL-2R beta, and IL-2R gamma chains, the last of which is also used in the receptors for IL-4, IL-7, and IL-9. Stimulation with IL-2 induces the tyrosine phosphorylation and activation of the Janus kinases Jak1 and Jak3. Jak1 and Jak3 were found to be selectively associated with the "serine-rich" region of IL-2R beta and the carboxyl-terminal region of IL-2R gamma, respectively. Both regions were necessary for IL-2 signaling. Furthermore, Jak3-negative fibroblasts expressing reconstituted IL-2R became responsive to IL-2 after the additional expression of Jak3 complementary DNA. Thus, activation of Jak1 and Jak3 may be a key event in IL-2 signaling.
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Sigma-Aldrich
Jak1 active human, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)