581616-U
Ascentis® Phenyl HPLC Column
5 μm particle size, L × I.D. 15 cm × 4.6 mm
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About This Item
Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Prodotti consigliati
Materiali
stainless steel column
Livello qualitativo
agenzia
suitable for USP L11
Nome Commerciale
Ascentis®
Caratteristiche
endcapped
Produttore/marchio commerciale
Ascentis®
Confezionamento
1 ea of
Grado di funzionalizzazione
19% Carbon loading
Parametri
≤70 °C temp. range
400 bar pressure (5801 psi)
tecniche
HPLC: suitable
LC/MS: suitable
Lungh. × D.I.
15 cm × 4.6 mm
Area superficiale
450 m2/g
Copertura della superficie
5.2 μmol/m2
Impurezze
<5 ppm metals
Matrice
fully porous particle
silica gel high purity, spherical
Gruppo funzionale matrice
phenyl phase
Dimensione particelle
5 μm
Dimensione pori
100 Å
operating pH range
2-8
applicazioni
food and beverages
Tecnica di separazione
reversed phase
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Descrizione generale
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
The Ascentis Phenyl provides superior separations in reversed-phase mode including 100% aqueous conditions. It may also be used in HILIC/ANP (aqueous normal phase) mode and shows low UV/MS bleed for gradient applications.
The Ascentis Phenyl provides superior separations in reversed-phase mode including 100% aqueous conditions. It may also be used in HILIC/ANP (aqueous normal phase) mode and shows low UV/MS bleed for gradient applications.
Caratteristiche e vantaggi
- 100% Aqueous Compatible
- ANP/HILIC and reversed-phase
- Low UV/MS bleed for gradient applications
- Alternate selectivity
Note legali
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Precolonna
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C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
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