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Key Documents

SAB4200255

Sigma-Aldrich

Anti-PP2A subunit B isoform B56-δ antibody, Mouse monoclonal

clone H5D12, purified from hybridoma cell culture

Sinonimo/i:

Anti-PP2A B subunit isoform PR61-δ, Anti-PP2A B subunit isoform R5-δ, Anti-PPP2R5D, Anti-Protein phosphatase 2, regulatory subunit B′,δ, Anti-Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, δ isoform

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.44

Origine biologica

mouse

Coniugato

unconjugated

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

H5D12, monoclonal

Forma fisica

buffered aqueous solution

PM

antigen ~72 kDa

Reattività contro le specie

human, rat

Concentrazione

~1.0 mg/mL

tecniche

immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 2.5-5.0 μg/mL using whole extracts of human U-2-OS or rat Rat2 cells.

Isotipo

IgG1

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... PPP2R5D(5528)

Descrizione generale

Monoclonal Anti-PP2A subunit B isoform B56-δ (mouse IgG1 isotype) is derived from the hybridoma H5D12 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein. PP2A subunit B isoform B56 d (PPP2R5D) belongs to the phosphatase PP2A regulatory subunit B family. PP2A subunit B isoform B56 d (PPP2R5D) encodes a δ isoform of the regulatory subunit B56 subfamily. It is a member of phosphatase PP2A regulatory subunit B family. The core enzyme is a dimer composed of a catalytic subunit (C) and a constant regulatory subunit (A), that associates with a regulatory subunit (B).

Specificità

The antibody recognizes human and rat PP2A B subunit isoform B56-δ. The antibody does not cross-react with PP2A-B55, PP2A-B56-α, PP2A-B56-β, PP2A-B56-γ or PP2A-B56-ε.

Immunogeno

fusion protein corresponding to the N-terminus of human PP2A B subunit isoform B56δ.

Applicazioni

Monoclonal Anti-PP2A subunit B isoform B56-δ antibody produced in mouse has been used in immunoblotting.

Azioni biochim/fisiol

PP2A is a serine/threonine phosphatase that downregulates the MAP kinase pathway and subsequently modulates mitogenic signaling. This phosphatase also mediates cellular invasion, localization of Sgo1 at centromeres, cell movement, Ras-1 activation, and chromosome segregation. PP2A protein complex consists of a dimeric core containing subunits A and C; which in turn associates with the regulatory subunit B. PP2A subunit B is involved in the degranulation of mast cells .
Protein phosphatase 2A (PP2A) is implicated in the negative control of cell growth and division.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Regulation of the Cell Cycle by Protein Phosphatase 2A in Saccharomyces cerevisiae
Jiang Y
Microbiology and Molecular Biology Reviews, 70, 440-440 (2006)
Sunanda Basu
Current protein & peptide science, 12(1), 3-11 (2010-12-31)
Cell motility is a very critical phenomenon that plays an important role in the development of eukaryotic organisms. One of the well studied cell motility phenomena is chemotaxis, which is described as a directional movement of cell in response to
Gregory Kranias et al.
Cellular signalling, 22(12), 1882-1890 (2010-08-07)
Asthma is characterised by antigen-mediated mast cell degranulation resulting in secretion of inflammatory mediators. Protein phosphatase 2A (PP2A) is a serine/threonine protein phosphatase composed of a catalytic (PP2A-C) subunit together with a core scaffold (PP2A-A) subunit and a variable, regulatory
Sean T DeGrande et al.
The Journal of biological chemistry, 288(2), 1032-1046 (2012-12-04)
Kinase/phosphatase balance governs cardiac excitability in health and disease. Although detailed mechanisms for cardiac kinase regulation are established, far less is known regarding cardiac protein phosphatase 2A (PP2A) regulation. This is largely due to the complexity of the PP2A holoenzyme
Zhanyun Tang et al.
Developmental cell, 10(5), 575-585 (2006-04-04)
Loss of sister-chromatid cohesion triggers chromosome segregation in mitosis and occurs through two mechanisms in vertebrate cells: (1) phosphorylation and removal of cohesin from chromosome arms by mitotic kinases, including Plk1, during prophase, and (2) cleavage of centromeric cohesin by

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