GE28-9893-34
HiLoad® 26/600 Superdex® 75 pg
Cytiva 28-9893-34, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size
About This Item
Prodotti consigliati
Materiali
glass column
glass fiber (polypropylene reinforced)
Durata
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
PM
3000-70000 Da (globular proteins)
Confezionamento
pkg of 1 ea
Produttore/marchio commerciale
Cytiva 28-9893-34
Parametri
<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa (42 psi)
Volume del letto
320 mL
Lungh. × diam. int. colonna
60 cm × 26 mm
Matrice
agarose dextran composite
dextran-agarose particle platform
polymeric
Gruppo funzionale matrice
dextran-agarose phase
dextran-agarose phase
Dimens. media delle particelle
34 μm
cleaning
1-14
working range
3-12
Tecnica di separazione
size exclusion (SEC)
Categorie correlate
Descrizione generale
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
Caratteristiche e vantaggi
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
Compatibilità
Stoccaggio e stabilità
Risultati analitici
Note legali
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Articoli
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
Protocolli
Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
This page shows how to refold proteins using gel filtration and products from GE Healthcare.
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.
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