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G0774

Sigma-Aldrich

Glycerokinase from Bacillus stearothermophilus

buffered aqueous solution, ≥75 units/mg protein (biuret)

Sinonimo/i:

ATP:glycerol 3-phosphotransferase, Glycerol Kinase

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About This Item

Numero CAS:
9030-66-4
Classificazione EC (Enzyme Commission):
2.7.1.30 (BRENDA, IUBMB)
Numero CE:
232-862-0
Numero MDL:
MFCD00131205
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

Origine biologica

Bacillus sp. (Bacillus steaarothermophilus)

Saggio

≥5.0 mg protein/mL (biuret)

Forma fisica

buffered aqueous solution

Attività specifica

≥75 units/mg protein (biuret)

Condizioni di stoccaggio

dry at room temperature

Colore

beige

applicazioni

life science and biopharma

Temperatura di conservazione

2-8°C

Informazioni sul gene

Bacillus sp. ... glpK(89613566)

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Descrizione generale

Research area: Cell Signaling

Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.

Applicazioni

Glycerokinase from Bacillus stearothermophilus has been used in steady-state kinetics studies.

Azioni biochim/fisiol

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.

Definizione di unità

One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.

Stato fisico

Stabilized solution in Tris buffer, pH 7.3

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Purification and properties of glycerol kinase from Escherichia coli.
S I Hayashi et al.
The Journal of biological chemistry, 242(5), 1030-1035 (1967-03-10)
N Zwaig et al.
Journal of bacteriology, 102(3), 753-759 (1970-06-01)
The activity of glycerol kinase is rate-limiting in the metabolism of glycerol by cells of Escherichia coli. A mutant strain producing a glycerol kinase resistant to inhibition by fructose-1,6-diphosphate grows faster than its wild-type parent on glycerol as the sole
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The British journal of nutrition, 107(11), 1694-1706 (2011-10-15)
Animal and human studies suggest that a malleable protein matrix (MPM) from whey decreases plasma lipid concentrations and may positively influence other components of the metabolic syndrome such as glucose metabolism and blood pressure (BP). The primary objective of this
Daria V Cherkashina et al.
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Abstract Effects of stem and progenitor cells or their compounds on recipient cells are investigated intensively today. In spite of this, their ability to interact with native cells and the final targets affected by them, particularly biochemical parameters that characterize
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International journal of molecular medicine, 29(6), 1121-1126 (2012-03-20)
Second generation n3-PUFA-depleted rats represent a good animal model of metabolic syndrome as they display several features of the disease such as liver steatosis, visceral obesity and insulin resistance. The goal of our study was to investigate the influence of
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