T98G Cell Line human

92090213

• Sinonimo/i: T 98 G Cells, T-98G Cells, T98-G Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human brain
• Descrizione: Human Caucasian glioblastoma
• Modalità di accrescimento: Adherent
• Cariotipo: Hyperpentaploid, modal no. 128-132
• Morfologia: Not specified
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian glioblastoma

Descrizione della linea cellulare

Derived from glioblastoma multiform tumour from a 61-year-old Caucasian male. Indefinite lifespan and anchorage independent but can enter viable G1 arrested state when deprived of serum.

Applicazioni

Studies on the mechanism for cessation of proliferation in quiescent cells and cells synchronised in G1 phase.

T98G has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study the efficiency of empty spiracles homeobox 2 (EMX2) in suppressing glioblastoma.

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 13
D5S818: 10,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 17,20

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells may become anchorage-independent if deprived of serum.

Altre note

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