KNRK

87061001, rat kidney, Epithelial-like

• Codice UNSPSC: 41106514

Proprietà

• product name: KNRK, 87061001
• Origine biologica: rat kidney
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial-like
• Prodotti: Growth factors
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Normal rat kidney, Kirsten MSV transformed

Descrizione della linea cellulare

Derived from normal rat kidney transformed by Kirsten sarcoma virus. Cells do not produce virus but carry the virus genome. In serum free medium the cell line produces growth factors capable of stimulating quiescent cells to grow in monolayers or in soft agar.

Applicazioni

Transformation, growth factors and virus studies: Kirsten MSV

Terreno di coltura

DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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