CRFK (BVD Ag negative)

86093002, feline kidney, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: CRFK (BVD Ag negative), 86093002
• Origine biologica: feline kidney
• Modalità di accrescimento: Adherent
• Cariotipo: Hypodiploid, modal no 36
• Morfologia: Epithelial
• Prodotti: Carcinoembryonic antigen (CEA)
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Cat kidney

Descrizione della linea cellulare

CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.

Applicazioni

Crandell feline kidney cells (CRFK) (BVD Ag negative) have been used in viral studies.

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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