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Key Documents

A1047

Sigma-Aldrich

Anti-IgG di topo (specifico per catena γ)−fosfatasi alcalina

affinity isolated antibody, buffered aqueous solution

Sinonimo/i:

Goat Anti-Mouse IgG (γ-chain specific)−AP

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

goat

Ricombinante

expressed in goat

Coniugato

alkaline phosphatase conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

mouse

tecniche

direct ELISA: 1:7,000-1:21,000
western blot: suitable

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

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Descrizione generale

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibody associates with mouse IgG (gamma-chain specific).

Immunogeno

purified mouse IgG

Applicazioni

Anticorpi anti Trypanosoma cruzi sono stati rilevati nei sieri di topi infettati grazie al test ELISA con un anticorpo di capra anti IgG di topo coniugato con fosfatasi alcalina specifico per catena. Le piastre sono state rivestite con antigene di T. cruzi e bloccate con BSA/PBS all′1% prima dell′incubazione con l′anticorpo coniugato.
For detection of IgG antibodies in mouse sera and saliva following S. gordonii infection, ELISA assays were performed using anti-mouse IgG alkaline phosphatase antibody at 1:20000. This antibody did not detect IgA antibodies.
Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibodies can be used for western blot assays,.

Stato fisico

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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In order to investigate rodent host specificity of European hantaviruses, experimental infection of colonized and wild-trapped rodents was performed. In addition to the natural rodent reservoir, Clethrionomys glareolus, Puumala hantavirus (PUUV) could infect colonized Microtus agrestis and Lemmus sibiricus, but
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