MABN48

Anti-UCHL1 Antibody, clone MCA-BH7

clone MCA-BH7, from mouse

• Sinonimo/i: Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: MCA-BH7, monoclonal
• Reattività contro le specie: human, bovine, rat
• tecniche: immunofluorescence: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_004172
• N° accesso UniProt: P09936
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... UCHL1(7345)

Descrizione

Descrizione generale

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), a thiol protease belonging to the peptidase C12 family, is involved in the processing of ubiquitin precursors and of ubiquitinated proteins. UCH-L1 recognizes and hydrolyzes peptide bonds at the C-termial glycine of ubiquitin. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Defects in UCH-L1 are the cause of Parkinson disease type 5.

Immunogeno

His-tagged recombinant protein corresponding to human UCHL1.

Applicazioni

Detect UCH-L1 using this mouse monoclonal antibody, Anti-UCHL1 Antibody, clone MCA-BH7 validated for use in western blotting & Immunofluorescence.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected UCHL1 in 10 µg of human brain tissue lysate.

Immunofluorescence Analysis: A 1:2,000 dilution from a representative lot detected UCHL1 in rat brain spinal cord tissue (Gerry Shaw. EnCor Biotechnology, Inc., Gainesville, Florida).

Qualità

Evaluated by Western Blotting in rat brain spinal cord tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected UCHL1 in 10 µg of rat brain tissue lysate.

Descrizione del bersaglio

~25 kDa observed

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
Rat brain spinal cord tissue lysate.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable