ABS65

Anti-CPT1A Antibody

serum, from rabbit

• Sinonimo/i: Carnitine O-palmitoyltransferase 1, liver isoform, CPT1-L, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPTI-L, Carnitine palmitoyltransferase 1A
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: rat, mouse
• tecniche: western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_001867
• N° accesso UniProt: P50416
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Cpt1A(12894)

Descrizione

Descrizione generale

Carnitine palmitoyltransferase 1A (CPT1A, CPT1-L, or L-CPT1) is an hepatic enzyme that, together with CPT2 and carnitine-acylcarnitine translocase, mediates the oxidation of long chain fatty acids to produce energy, in periods of fasting. CPT1A is localized in the outer mitochondrial membrane and catalyzes the addition of carnitine to long-chain fatty acids thereby enabling effective transport of these molecules to the inner mitochondrial membrane where they are metabolized. CPT1A may function as part of a complex consisting of the acyl-CoA synthetase (ACSL) and the voltage-dependent anion channel (VDAC) and is regulated by malonyl-CoA. Deficiencies in CPT1A result in hypoketotic hypoglycemia.

Immunogeno

GST-tagged recombinant protein corresponding to rat CPT1A.

Applicazioni

Detect CPT1A using this Anti-CPT1A Antibody validated for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Qualità

Evaluated by Western Blot in mouse liver tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected CPT1A on 10 µg of mouse liver tissue lysate.

Descrizione del bersaglio

~78 kDa observed. An uncharacterized band appears at ~32 kDa in some lysates. The calculated molecular weight is 88 kDa, however CPT1A has been shown as a ~78 kDa band in western blots (Tan, L., et al (2011). J Inherit Metab Dis. 34:443–447.)

Stato fisico

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
Mouse liver tissue lysate

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1