AB5208
Anti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain
Chemicon®, from rabbit
Sinonimo/i:
Nav1.3, SCN3A
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
rat, mouse
Produttore/marchio commerciale
Chemicon®
tecniche
immunohistochemistry: suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... SCN3A(6328)
Specificità
Recognizes Nav1.3 (type III a subunit of VGSC, Scn3a). Does not cross react with any other sodium channel antigens tested so far.
Immunogeno
Purified peptide of alpha subunit (amino acids 511-524) of rat Nav1.3
( type III voltage-gated sodium channel VGSC)
( type III voltage-gated sodium channel VGSC)
Applicazioni
All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).
Western blot: 1:200 using ECL on newborn rat brain membranes.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Immunohistochemistry: on fixed frozen sections.
Optimal working dilutions must be determined by the end user.
Western blot: 1:200 using ECL on newborn rat brain membranes.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Immunohistochemistry: on fixed frozen sections.
Optimal working dilutions must be determined by the end user.
Detect Sodium Channel using this Anti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain validated for use in IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Stato fisico
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 50 mL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Stoccaggio e stabilità
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Risultati analitici
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 0.75 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 0.75 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Frank H Yu et al.
Nature neuroscience, 9(9), 1142-1149 (2006-08-22)
Voltage-gated sodium channels (Na(V)) are critical for initiation of action potentials. Heterozygous loss-of-function mutations in Na(V)1.1 channels cause severe myoclonic epilepsy in infancy (SMEI). Homozygous null Scn1a-/- mice developed ataxia and died on postnatal day (P) 15 but could be
Sebastian K G Maier et al.
Circulation, 109(11), 1421-1427 (2004-03-10)
Voltage-gated sodium channels composed of pore-forming alpha and auxiliary beta subunits are responsible for the rising phase of the action potential in cardiac muscle, but their localizations have not yet been clearly defined. Immunocytochemical studies show that the principal cardiac
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