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Sigma-Aldrich

PIP5-Kinase HTRF Assay

The PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 384 well format. Suitable for screening of potential inhibitors of these enzymes.

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About This Item

Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.54

Livello qualitativo

Reattività contro le specie

mouse, human

Produttore/marchio commerciale

HTRF®
Upstate®

tecniche

activity assay: suitable (kinase)

Metodo di rivelazione

fluorometric (HTRF)

Condizioni di spedizione

dry ice

Applicazioni

Research Category
Apoptosis & Cancer
Research Sub Category
Oncology
The PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 384 well format. Suitable for screening of potential inhibitors of these enzymes.
Used to detect/quantify: PIP5 Kinase

Azioni biochim/fisiol

Target Sub-Family: PI3K/PI4K

Confezionamento

Kit capacity: 384 wells

Componenti

Reaction Buffer (4X)

DM A Detection Reagent

DM C Detection Reagent

DM E Detection Reagent

Phosphatidylinositol 4-phosphate (1mM)

Phosphatidylserine (1mM)

Stop Reagent D

Stoccaggio e stabilità

12 months at -70°C

Note legali

HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

10-13 - German Storage Class 10 to 13


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Yuxun Zhang et al.
The Journal of biological chemistry, 292(24), 10239-10249 (2017-05-02)
SIRT5 is a lysine desuccinylase known to regulate mitochondrial fatty acid oxidation and the urea cycle. Here, SIRT5 was observed to bind to cardiolipin via an amphipathic helix on its N terminus. In vitro, succinyl-CoA was used to succinylate liver
Marco Spinazzi et al.
Nature protocols, 7(6), 1235-1246 (2012-06-02)
The assessment of mitochondrial respiratory chain (RC) enzymatic activities is essential for investigating mitochondrial function in several situations, including mitochondrial disorders, diabetes, cancer, aging and neurodegeneration, as well as for many toxicological assays. Muscle is the most commonly analyzed tissue

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