322133

DSP, Water-Soluble

• Sinonimo/i: 3,3-Dithio- bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP, 3,3-Dithio-bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP
• Codice UNSPSC: 41116133
• NACRES: NA.24

Proprietà

• Saggio: ≥94% (HPLC)
• Livello qualitativo: 100
• Forma fisica: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated
• Colore: off-white to light tan
• Solubilità: water: 6 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Bifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH₄, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups.

Bifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH₄, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups. Chemical crosslinkers are used to covalently modify proteins for studying ligand receptor interactions, conformational changes in tertiary structure, or for protein labeling. Crosslinkers are divided into homobifunctional crosslinkers, containing two identical reactive groups, or heterobifunctional crosslinkers, containing two different reactive groups. Water-soluble cross-linking reagents help to avoid the use of organic solvents such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) when preparing bioconjugates.

Attenzione

Toxicity: Irritant (B)

Ricostituzione

Following each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid DSP can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.

Altre note

Howard, A.D., et al. 1985. J. Biol. Chem.260, 10833.
Lee, W.T., and Conrad, D.H. 1985. J. Immunol.134, 518.
D′Souza, S.E., et al. 1988. J. Biol. Chem.263, 3943.
Yamada, H., et al. 1981. Biochem.20, 4836.
Annunziato, M.E., et al. 1993. Bioconjugate Chem.4, 212.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable