05-784R

Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal

clone 58-10C-31, from rabbit

• Sinonimo/i: insulin receptor substrate 1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 58-10C-31, monoclonal
• Reattività contro le specie: human, rat, mouse
• tecniche: immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_0371
• N° accesso UniProt: P35570
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... IRS1(3667)

Descrizione

Descrizione generale

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. It is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer.

Specificità

Antibody recognizes the C-terminus of IRS1.

Immunogeno

Epitope: C-terminus

KLH- conjugated - linear peptide corresponding to the C-terminus of rat IRS1.

Applicazioni

Detect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP.

Research Category
Metabolism

Research Sub Category
Insulin/Energy Signaling

Western Blot (SNAP ID) Analysis: 0.1 µg/mL from a previous lot detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6 and MCF7 cell lysates.

Immunoprecipitation Analysis: 1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa.

Qualità

Evaluated by Western Blot in 3T3/A31, 3T3/L1, L6 or MCF7 cell lysates.

Western Blot Analysis: 0.1 µg/mL of this antibody detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates.

Descrizione del bersaglio

~ 160 kDa

Stato fisico

Format: Purified

Protein A purified

Purified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% glycerol.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Risultati analitici

Control
3T3/A31, 3T3/L1, L6, or MCF7 cell lysates

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable