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Merck

PCR in as Little as 15 Minutes!

KOD One™ PCR Master Mix for
Ultra-Fast, High Fidelity PCR

KOD One™ PCR Master Mix

KOD One™ PCR Master Mix is the right choice for ultra-fast PCR. It offers the fastest elongation rates available for high fidelity polymerases without compromising on the yield and specificity. This convenient, premixed, ready-to-use solution offers an elongation rate of up to 5 seconds per kb and exhibits approximately 80-fold higher fidelity than Taq DNA polymerase. Additionally, this flexible master mix will easily fit right into your PCR workflow with its ability to amplify directly from crude samples and use primers containing both uracil and inosine. Choose KOD One™ PCR Master Mix and efficiently amplify your samples with confidence.

Get a 30% discount on KOD One™ PCR Master Mix. Click here for the code. See what your fellow colleagues are saying about KOD One™ PCR reagents below and let us know your thoughts by leaving a review!

Learn more about how KOD One™ PCR Master Mix can easily fit into your PCR workflow.

KOD One™ PCR Master Mix

Benefits

The KOD One™ PCR Master Mix formulation offers the following advanced features:

  • Ready-to-Use
  • Added Flexibility
  • High Fidelity
  • High Efficiency
  • Crude Sample PCR
  • High Specificity
  • Primers with inosine (dI) and uracil (dU)

Applications

This product has a variety of applications such as:

  • DNA/cDNA amplification
  • Direct PCR
  • Colony PCR
  • NGS library amplification
  • PCR amplification to detect site-directed mutagenesis

PCR Master Mix and Polymerase Cross Reference Guide

KOD One™ PCR Master Mix Customer Testimonials

Aleksandar Vještica
Assistant Professor

Organization: University of Lausanne
Field: Academia / University

"Thanks to its high processivity, KOD One™ Master Mix allowed us to quickly clone plasmids using only overlapping PCRs. This approach did not require restriction enzyme digestions of cloning inserts and thus considerably simplified the cloning protocol and saved us time. "

Review date: 3 December 2021
KOD One™ PCR Master Mix

Blanco Lago Solis
Ph.D. Student

Organization: University of Geneva
Field: Academia / University

"I successfully amplified a 14kb fragment in 2h15 vs 4h30 with Phusion or Q5."

KOD One™ PCR Master Mix

Maria Cristina Gambetta
Assistant Professor

Organization: University of Lausanne
Field: Academia / University

"I was caught red-handed in my impatience. I asked around and indeed got an aliquot of KOD One™ master mix promising to give it back. It worked wonderfully! I guess this really is a miraculous polymerase. So, we went ahead and ordered master mix to not waste time, as experiments are urgent."

Review date: 16 December 2021
KOD One™ PCR Master Mix

Dr. Michael Taschner
Senior Scientist

Organization: University of Lausanne
Field: Academia / University

"I received a free sample of KOD One™ PCR master mix to test. I was amazed by the speed, processivity, and accuracy of PCRs carried out on complicated templates. No other product I used in the past was able to amplify a product of >12 kb in record time from ‘quick-and-dirty’ yeast genomic DNA (purified according to [Lōoke et al, BioTechniques, 2011]. I fully recommend this product for difficult PCR cases."

Review date: 17 December 2021
KOD One™ PCR Master Mix

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