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RPMI-1640 Media Formulation

RPMI-1640 Medium Composition

ComponentR0883
[1x] g/L
R1145
[10x] g/L
R1383
g/L
R1780
[1x] g/L
R2405
[1x] g/L
R6504
g/L
R8758
[1x] g/L
Inorganic Salts
Calcium Nitrate • 4H2O0.110.10.10.10.10.1
Magnesium Sulfate (anhydrous)0.048840.48840.048840.048840.048840.048840.04884
Potassium Chloride0.440.40.40.40.40.4
Sodium Bicarbonate2222
Sodium Chloride66066666
Sodium Phosphate Dibasic (anhydrous)0.880.80.80.80.80.8
Amino Acids
L-Alanyl-L-Glutamine0.4344
L-Arginine0.220.20.20.20.2
L-Asparagine (anhydrous)0.050.50.050.050.050.050.05
L-Aspartic Acid0.020.20.020.020.020.020.02
L-Cystine • 2HCl0.06520.6520.06520.06520.06520.06520.0652
L-Glutamic Acid0.020.20.020.020.020.020.02
L-Glutamine0.30.30.30.3
Glycine0.010.10.010.010.010.010.01
L-Histidine0.0150.150.0150.0150.0150.0150.015
Hydroxy-L-Proline0.020.20.020.020.020.020.02
L-Isoleucine0.050.50.050.050.050.050.05
L-Leucine0.050.50.050.050.050.05
L-Lysine • HCl0.040.40.040.040.040.04
L-Methionine0.0150.150.0150.0150.0150.0150.015
L-Phenylalanine0.0150.150.0150.0150.0150.0150.015
L-Proline0.020.20.020.020.020.020.02
L-Serine0.030.30.030.030.030.030.03
L-Threonine0.020.20.020.020.020.020.02
L-Tryptophan0.0050.050.0050.0050.0050.0050.005
L-Tyrosine • 2Na • 2H2O0.028830.28830.028830.028830.028830.028830.02883
L-Valine0.020.20.020.020.020.020.02
Vitamins
D-Biotin0.00020.0020.00020.0020.0020.00020.0002
Choline Chloride0.0030.030.0030.0030.0030.0030.003
Folic Acid0.0010.0010.0010.0010.0010.001
myo-Inositol0.0350.350.0350.0350.0350.0350.035
Niacinamide0.0010.010.0010.0010.0010.0010.001
p-Aminobenzoic Acid0.0010.010.0010.0010.0010.0010.001
D-Pantothenic Acid (hemicalcium)0.000250.00250.000250.000250.000250.000250.00025
Pyridoxine • HCl0.0010.010.0010.0010.0010.0010.001
Riboflavin0.00020.0020.00020.00020.00020.00020.0002
Thiamine • HCl0.0010.010.0010.0010.0010.0010.001
Vitamin B120.0000050.000050.0000050.0000050.0000050.0000050.000005
Other
D-Glucose2202222
Glutathione (reduced)0.0010.010.0010.0010.0010.0010.001
Phenol Red • Na0.00530.0530.00530.00530.00530.0053
Add
L-Glutamine0.30.3 at 1×
Sodium Bicarbonate2.0 at 1×22

RPMI-1640 Medium Dutch Modification

ComponentR7638
[1x] g/L
Inorganic Salts
Calcium Nitrate • 4H2O0.1
Magnesium Sulfate (anhydrous)0.04884
Potassium Chloride0.4
Sodium Bicarbonate1
Sodium Chloride6.4
Sodium Phosphate Dibasic (anhydrous)0.8
Amino Acids
L-Arginine0.2
L-Asparagine (anhydrous)0.05
L-Aspartic Acid0.02
L-Cystine • 2HCl0.0652
L-Glutamic Acid0.02
Glycine0.01
L-Histidine0.015
Hydroxy-L-Proline0.02
L-Isoleucine0.05
L-Leucine0.05
L-Lysine • HCl0.04
L-Methionine0.015
L-Phenylalanine0.015
L-Proline0.02
L-Serine0.03
L-Threonine0.02
L-Tryptophan0.005
L-Tyrosine • 2Na • 2H2O0.02883
L-Valine0.02
Vitamins
D-Biotin0.0002
Choline Chloride0.003
Folic Acid0.001
myo-Inositol0.035
Niacinamide0.001
p-Aminobenzoic Acid0.001
D-Pantothenic Acid (hemicalcium)0.00025
Pyridoxine • HCl0.001
Riboflavin0.0002
Thiamine • HCl0.001
Vitamin B120.000005
Other
D-Glucose2
Glutathione (reduced)0.001
HEPES4.77
Phenol Red • Na0.0053
Add
L-Glutamine0.3

RPMI-1640 Medium HEPES Modification

ComponentR4130
g/L
R5886
[1x] g/L
R8005
g/L
Inorganic Salts
Calcium Nitrate • 4H2O0.10.10.1
Magnesium Sulfate (anhydrous)0.048840.048840.04884
Potassium Chloride0.40.40.4
Sodium Bicarbonate2
Sodium Chloride665.9
Sodium Phosphate Dibasic (anhydrous)0.80.80.8
Amino Acids
L-Arginine0.20.20.2
L-Asparagine (anhydrous)0.050.050.05
L-Aspartic Acid0.020.020.02
L-Cystine • 2HCl0.06520.06520.0652
L-Glutamic Acid0.020.020.02
L-Glutamine0.30.3
Glycine0.010.010.01
L-Histidine0.0150.0150.015
Hydroxy-L-Proline0.020.020.02
L-Isoleucine0.050.050.05
L-Leucine0.050.050.05
L-Lysine • HCl0.040.040.04
L-Methionine0.0150.0150.015
L-Phenylalanine0.0150.0150.015
L-Proline0.020.020.02
L-Serine0.030.030.03
L-Threonine0.020.020.02
L-Tryptophan0.0050.0050.005
L-Tyrosine • 2Na • 2H2O0.028830.028830.02883
L-Valine0.020.020.02
Vitamins
D-Biotin0.00020.00020.0002
Choline Chloride0.0030.0030.003
Folic Acid0.0010.0010.001
myo-Inositol0.0350.0350.035
Niacinamide0.0010.0010.001
p-Aminobenzoic Acid0.0010.0010.001
D-Pantothenic Acid (hemicalcium)0.000250.000250.00025
Pyridoxine • HCl0.0010.0010.001
Riboflavin0.00020.00020.0002
Thiamine • HCl0.0010.0010.001
Vitamin B120.0000050.0000050.000005
Other
D-Glucose224.5
Glutathione (reduced)0.0010.0010.001
HEPES5.965.963.5745
Phenol Red • Na0.00530.00530.0053
Add
L-Glutamine0.3
Sodium Bicarbonate22

RPMI-1640 Medium Modified

ComponentR7388
[1x] g/L
R7509
[1x] g/L
R7513
[1x] g/L
R8755
g/L
Inorganic Salts
Calcium Nitrate • 4H2O0.10.10.10.1
Magnesium Sulfate (anhydrous)0.048840.048840.048840.04884
Potassium Chloride0.40.40.40.4
Sodium Bicarbonate22
Sodium Chloride6666
Sodium Phosphate Dibasic (anhydrous)0.80.80.80.8
Amino Acids
L-Arginine0.20.20.20.2
L-Asparagine (anhydrous)0.050.050.050.05
L-Aspartic Acid0.020.020.020.02
L-Cystine • 2HCl0.06520.06520.0652
L-Glutamic Acid0.020.020.020.02
L-Glutamine0.30.3
Glycine0.010.010.010.01
L-Histidine0.0150.0150.0150.015
Hydroxy-L-Proline0.020.020.020.02
L-Isoleucine0.050.050.050.05
L-Leucine0.050.050.050.05
L-Lysine • HCl0.040.040.040.04
L-Methionine0.0150.0150.015
L-Phenylalanine0.0150.0150.0150.015
L-Proline0.020.020.020.02
L-Serine0.030.030.030.03
L-Threonine0.020.020.020.02
L-Tryptophan0.0050.0050.0050.005
L-Tyrosine • 2Na • 2H2O0.028830.028830.028830.02883
L-Valine0.020.020.020.02
Vitamins
D-Biotin0.00020.00020.00020.0002
Choline Chloride0.0030.0030.0030.003
Folic Acid0.0010.0010.0010.001
myo-Inositol0.0350.0350.0350.035
Niacinamide0.0010.0010.0010.001
p-Aminobenzoic Acid0.0010.0010.0010.001
D-Pantothenic Acid (hemicalcium)0.000250.000250.000250.00025
Pyridoxine • HCl0.0010.0010.0010.001
Riboflavin0.00020.00020.00020.0002
Thiamine • HCl0.0010.0010.0010.001
Vitamin B120.0000050.0000050.0000050.000005
Other
D-Glucose2222
Glutathione (reduced)0.0010.0010.0010.001
HEPES4.77
Phenol Red • Na0.00530.0053
Add
L-Cystine • 2HCl0.0652
L-Glutamine0.30.3
L-Methionine0.015
Sodium Bicarbonate22

RPMI-1640 Medium Auto-Mod™ Modified for Autoclaving

ComponentR7755
g/L
Inorganic Salts
Calcium Nitrate • 4H2O0.1
Magnesium Sulfate (anhydrous)0.04884
Potassium Chloride0.4
Sodium Chloride6
Sodium Phosphate Dibasic (anhydrous)0.8
Succinic Acid • 6H2O • Na0.1
Succinic Acid (free acid)0.075
Amino Acids
L-Arginine0.2
L-Asparagine (anhydrous)0.05
L-Aspartic Acid0.02
L-Cystine • 2HCl0.0652
L-Glutamic Acid0.02
Glycine0.01
L-Histidine0.015
Hydroxy-L-Proline0.02
L-Isoleucine0.05
L-Leucine0.05
L-Lysine • HCl0.04
L-Methionine0.015
L-Phenylalanine0.015
L-Proline0.02
L-Serine0.03
L-Threonine0.02
L-Tryptophan0.005
L-Tyrosine0.02
L-Valine0.02
Vitamins
D-Biotin0.0002
Choline Bitartrate0.00544
Folic Acid0.001
myo-Inositol0.035
Niacinamide0.001
p-Aminobenzoic Acid0.001
D-Pantothenic Acid (hemicalcium)0.00025
Pyridoxine • HCl0.001
Riboflavin0.0002
Thiamine • HCl0.001
Vitamin B120.000005
Other
D-Glucose2
Glutathione (reduced)0.001
Phenol Red • Na0.00318
Add
L-Glutamine0.3
Sodium Bicarbonate2
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