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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Diethyl ether, ACS reagent, ≥98.0%, contains ≤2% ethanol and ≤10ppm BHT as inhibitor
Sigma-Aldrich
Toluene, ACS reagent, ≥99.5%
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Toluene, ACS reagent, ≥99.5%
Sigma-Aldrich
Diethyl ether, reagent grade, ≥98%, contains ≤2% ethanol and ≤10ppm BHT as inhibitor
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Toluene, ACS reagent, ≥99.5%
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N,N-Dimethylformamide, ACS reagent, ≥99.8%
Sigma-Aldrich
N,N-Dimethylformamide, ReagentPlus®, ≥99%
Supelco
Residual Solvent - Toluene, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Dimethylformamide, Pharmaceutical Secondary Standard; Certified Reference Material
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Diethyl ether, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor
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N,N-Diisopropylethylamine, 99.5%, biotech. grade
Sigma-Aldrich
Diethyl ether, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor
Sigma-Aldrich
Diethyl ether, suitable for HPLC, ≥99.9%, inhibitor-free
Sigma-Aldrich
N,N-Dimethylformamide, suitable for HPLC, ≥99.9%
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N,N-Dimethylformamide, biotech. grade, ≥99.9%
Supelco
Toluene, analytical standard
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2-Mercaptoethanol, ≥99.0%
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Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
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Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer
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Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
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Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
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Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
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Sodium chloride, BioXtra, ≥99.5% (AT)
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Sodium chloride, tablet
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2-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
SAFC
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