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GE28-9356-05

Sigma-Aldrich

HiPrep™ 26/60 Sephacryl® S-400 HR

Cytiva 28-9356-05, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

polypropylene

mol wt

20000-8000000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9356-05

parameter

<1.3 mL/min flow rate
<13 mL sample volume
0.15 Mpa (22 psi) (Over the Packed Bed During Operation)

bed volume

320 mL

column L × I.D.

60 cm × 26 mm

matrix

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

matrix active group

dextran-acrylamide phase

avg. part. size

50 μm

cleaning

2-13

working range

3-11

separation technique

size exclusion (SEC)

General description

HiPrep 26/60 Sephacryl S-400 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 ml sample volume. Sephacryl S-400 is optimized for separating polysaccarides, macromolecules with extended structures, and even small plasmids

Suitability

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
gel filtration columns for low to medium pressure ÄKTA systems+AE14

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Performing a Separation with Sephacryl®

This page shows how to perform a separation with a sephacryl column from GE Healthcare which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service