GE17-5111-01

HiTrap^® IGY Purification

Cytiva 17-5111-01, pack of 1 × 5 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: 2-mercaptopyridine
• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• packaging: pack of 1 × 5 mL
• manufacturer/tradename: Cytiva 17-5111-01
• storage condition: (20% Ethanol)
• parameter: <20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.); 42 psi
• bed size: 16 mm × 25 mm
• bed volume: 5 mL
• column I.D.: 16 mm
• matrix: highly cross-linked 6% agarose
• avg. part. size: 34 μm
• cleaning: 2-13
• working range: 3-11
• storage temp.: 2-30°C

Description

General description

HiTrap^® IgY Purification HP is a 5 mL column prepacked with Sepharose^™ High Performance for fast and easy purification of IgY from egg yolk.

Application

HiTrap^® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose^™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Features and Benefits

• Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
• Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose^™ High Performance.
• Recovery and purity of IgY are excellent.
• Can be operated with a liquid chromatography system such as ÄKTA^® design.
• The top and bottom frits are manufactured from porous polyethylene
• Connectors for usage with different chromatography systems and other equipment are included

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids