MAB352
Anti-Serotonin Antibody, clone YC5/45
culture supernatant, clone YC5/45, Chemicon®
Synonym(s):
5-HT
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rat
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
YC5/45, monoclonal
species reactivity
mouse, human, rat, lizard, rabbit, pig
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
isotype
IgG
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... HTR1A(3350)
Specificity
Recognizes the formaldehyde conjugate of serotonin. Without paraformaldehyde treatment, it cross-reacts in liquid phase with dopamine and tryptamine in hemagglutination tests. Shows no reactivity with catecholamine-containing neurons in formaldehyde fixed preparations. MAB352 reacts with serotonin in neurons and recognizes serotonergic sites in fixed tissue sections. By cell agglutination, no cross reactivity with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine or melatonin.
Immunogen
Serotonin conjugated to BSA.
Application
Anti-Serotonin Antibody, clone YC5/45 is an antibody against Serotonin for use in IH.
Immunohistochemistry at 1:100-1:200 for formaldehyde fixed tissue.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Physical form
Lyophilized Supernatant, preservative free.
Unpurified
Storage and Stability
Lyophilized antibody can be kept at 2-8°C for up to 3 months and should be kept at -20°C for long term storage. Reconstitute with 100μL sterile DI water. Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.
Analysis Note
Control
Brain tissue
Brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Reciprocal connections between subdivisions of the dorsal raphe and the nuclear core of the locus coeruleus in the rat.
Myung-A Kim, Hyun S Lee, Beob Y Lee, Barry D Waterhouse
Brain Research null
M Costa et al.
Neuroscience, 7(2), 351-363 (1982-02-01)
Immunoreactive nerve cell bodies and fibres in the intestine have been examined using three antibody preparations raised against 5-hydroxytryptamine. Cross reactivity studies indicate that the substance localized was an hydroxylated indoleamine. In the guinea-pig small intestine, nerve cell bodies were
Retrograde study of projections from the tuberomammillary nucleus to the dorsal raphe and the locus coeruleus in the rat.
Hyun S Lee, Beob Y Lee, Barry D Waterhouse
Brain Research null
Organisation and chemical neuroanatomy of the African elephant (Loxodonta africana) olfactory bulb.
Ngwenya A, Patzke N, Ihunwo AO, Manger PR.
Brain Structure &Amp; Function null
Analysis of gene function in the zebrafish retina.
Jarema Malicki, Hakryul Jo, Xiangyun Wei, Monica Hsiung, Zac Pujic
Methods null
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