ISO 6579-1 for Salmonella Detection in the Food Chain

The International Organization for Standardization (ISO) published the new EN ISO 6579-1 standard in 2017, which specifies a horizontal method for the detection of Salmonella spp. in the food production chain.

Like the preceding ISO 6579:2002+Amd 1:2007, the latest ISO 6579-1:2017 covers the testing of products intended for human consumption, animal feeding and environmental samples in food production and handling.

In addition, EN ISO 6579-1:2017 now includes testing of milk and milk products as well as samples from the primary production stage, such as animal feces, dust and boot socks.

Procedure for Salmonella detection according to EN ISO 6579-1

EN ISO 6579-1 at a Glance

• Part one (detection) of three-part EN ISO 6579 replaces EN ISO 6579:2004+Amd 1:2007
• EN ISO 6785:2007 (dairy products) method details now incorporated
• Samples from primary production stage added to scope
• Incubation temperature range for non-selective media extended to 34° – 38 °C
• Choice of RVS or MSRV agar for selective enrichment of food, animal feed and environmental samples
• Directions for choice of second isolation medium in addition to XLD agar
• Only one suspect colony needed for confirmation (if negative, 4 more to be tested)
• Optional: biochemical confirmation on well-isolated colony direct from selective plate
• β-galactosidase and indole tests now optional, Voges-Proskauer reaction omitted
• Detection of Salmonella Typhi and S. Paratyphi described
• Performance testing for the culture media in-depth

As a worldwide leading provider of a broad range of dehydrated granulated and ready-to-use culture media for food, beverages and water microbiology, we closely track and supports the development of relevant standards aimed at increasing consumer confidence and safety.

Our GranuCult™ granulated media, Readybag^® pre-weighed granulated media in ready-to-use bags, ReadyPlate™ & ReadyTube™ ready-to-use media are all compliant with EN ISO 11133:2014. All information regarding compliance with additional reference standards are displayed on the product label and in the product’s technical information available on our website.

Compliance to EN ISO 6579-1

We have implemented all the requirements described in EN ISO 6579-1. The following culture media and accessories described in ISO 6579-1 are available:

Ordering Information

Product	Pack Size	Catalog No.	Other Pack Sizes Available
GranuCult™ Buffered Peptone Water acc. ISO 6579, ISO 21528, ISO 22964, FDA-BAM and EP	500 g	1.07228.0500	5 kg, 25 kg
Readybag® Buffered Peptone Water acc. ISO 6579, ISO 21528, ISO 22964, FDA-BAM and EP, 5.7 g, irradiated	60 bags	1.02448.0060	60 bags x 29 g,
			35 bags x 86 g
ReadyTube™ 225 BPW ISO 6579, ISO 6887, ISO 21528, ISO 11290, FDA-BAM and EP	6 x 225 mL	1.46404.0006	6 x 1000 mL,
			20 x 9 mL
GranuCult™ RVS (RAPPAPORT-VASSILIADIS-Soya) Broth (Base) acc. ISO 6579	500 g	1.07700.0500
Novobiocin Sodium Salt	1 g	N6160-1-G	5 g, 25 g
ReadyTube™ 10 RVS Broth ISO 6579	20 x 10 mL	1.46694.0020
GranuCult™ MKTTn (MULLER KAUFFMANN Tetrathionate Novobiocin) Broth (Base) acc. ISO 6579	500 g	1.05878.0500
Potassium Iodide	250 g	1.05043.0250
Iodine resublimed	100 g	1.04761.0100
MSRV (Modified Semi-solid RAPPAPORT-VASSILIADIS) Medium (Base) acc. ISO 6579	500 g	1.09878.0500
MSRV Selective Supplement	10 x 1 vial	1.09874.0010
ReadyTube™ 12 MSRV Medium ISO 6579	100 x 12 mL	1.46622.0100
GranuCult™ XLD (Xylose Lysine Deoxycholate) agar acc. ISO 6579	500 g	1.05287.0500
ReadyPlate™ XLD Agar ISO 6579	20 x 90 mm	1.46751.0020
RAMBACH® Agar	4 x 250 mL	1.07500.0001	4 x 1000 mL, 1 x 50 L
RAMBACH® Agar ready-to-use	20 x 90 mm	1.46719.0020	100 x 90 mm
GranuCult™ Nutrient agar acc. ISO 6579, ISO 10273 and ISO 21528	500 g	1.05450.0500
Bismuth Sulfite Agar acc. WILSON-BLAIR	500 g	1.05418.0500
Triple Sugar Iron Agar	500 g	1.03915.0500
Urea Agar (Base) acc. CHRISTIANSEN	500 g	1.08492.0500
Urea GR for Analysis ACS, Reagent Ph Eur	500 g	1.08487.0500
MR-VP (Methyl Red-VOGES-PROSKAUER) Broth	500 g	1.05712.0500
KOVAC’S Indole Reagent	100 mL	1.09293.0100