HPLC Analysis of Free Fatty Acids on SUPELCOSIL™ LC-8
CONDITIONS
column
SUPELCOSIL LC-8, 15 cm × 4.6 mm I.D., 3 μm particles (58983)
mobile phase
acetonitrile:tetrahydrofuran: 0.1% phosphoric acid (50.4:21.6:28)
flow rate
1 mL/min
column temp.
35 °C
detector
UV, 215 nm
injection
10 μL
Description
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC