HPLC Analysis of Aflatoxins on Ascentis® Express C18
Materials
analytical column
CONDITIONS
column
Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase
[A] water; [B] methanol; [C] acetonitrile; (720:120:120, A:B:C) containing 0.780 g/L potassium bromide, 230 μL/L nitric acid
flow rate
0.400 mL/min
pressure
4568 psi (315 bar)
column temp.
35 °C
detector
FLD excitation = 360 nm, emission = 440 nm
injection
20 μL
sample
200 μL spiked 84:16 acetonitrile:di water in 880 μL di water, Spike concentrations: B1 = 16 ppb; B2 = 4 ppb; G1 = 16 ppb; B2 = 4 ppb
Description
Analysis Note
Aflatoxins are a complex of closely related toxins produced as secondary metablolites of fungi. They are found in grains and nuts such as corn, wheat, or peanuts. They are produced by aspergillus flavus and a. Parasiticus. Members of the group include aflatoxin b1, aflatoxin b2, aflatoxin g1, aflatoxin g2, aflatoxin m1, and aflatoxin m2.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany