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  • Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells.

Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells.

Journal of biomedical nanotechnology (2013-07-19)
Tianxun Gong, Malini Olivo, U S Dinish, Douglas Goh, Kien Voon Kong, Ken-Tye Yong
ABSTRACT

In this paper, we proposed the use of gold nanoparticles as plasmon scattering probes for dark-field multiplex imaging of live cancer cells. By carefully engineering the surfaces, aqueous dispersions of anti-EGFR antibody-conjugated gold nanospheres and gold nanorods are prepared. We demonstrated the receptor-mediated delivery of antibody conjugated gold nanospheres and gold nanorods into EGFR receptor-positive oral squamous cell carcinoma cell line by using darkfield microscopy technique. Our result suggests that gold nanospheres and gold nanorods formulations could provide up to 7 types of plasmon scattering probes based on their tunability range with a 100 nm spectral separation in absorption bands.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gold, powder, <45 μm, 99.99% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 0.25 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold coated glass slide, 99.999% (Au), layer thickness 1000 Å
Sigma-Aldrich
Gold coated glass slide, 99.999% (Au), layer thickness 100 Å