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  • Preparation and evaluation of natural scaffold materials for kidney regenerative applications.

Preparation and evaluation of natural scaffold materials for kidney regenerative applications.

Methods in molecular biology (Clifton, N.J.) (2013-03-16)
Monica A Serban, Toyin A Knight, Richard G Payne
ABSTRACT

Tissue engineering involves the concerted action of biomaterials, cells, and growth factors. Kidney -regeneration relies on the same combination of ingredients. Here, we describe an example of gelatin-based biomaterial preparation and its evaluation in the context of kidney biocompatibility and integration. This biomaterial manufacturing technique is simple, cost-effective, highly reproducible and the in vivo evaluation procedure highly informative on the biocompatibility and regenerative potential of the tested construct.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gelatin from bovine skin, gel strength ~225 g Bloom, Type B
Sigma-Aldrich
Gelatin from bovine skin, Type B, powder, BioReagent, suitable for cell culture