A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry.

A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry.

Journal of breath research (2008-09-01)

S Smith, H Burden, R Persad, K Whittington, B de Lacy Costello, N M Ratcliffe, C S Probert

ABSTRACT

First-void urine samples were obtained from 24 elderly, asymptomatic men (median age 62.9 years). The headspace above pH adjusted urine samples were extracted using a carboxen/polydimethylsiloxane solid phase micro-extraction fibre and the volatile organic compounds analysed by gas chromatography/mass spectrometry. A total of 147 compounds were identified in the headspace of urine. The acidified samples recorded a total of 92 compounds, 27 of which were ubiquitous, basified samples 70 compounds, with 12 ubiquitous and unmodified pH samples 49, with 6 ubiquitous. Five compounds were ubiquitous irrespective of pH: acetone, methylene chloride, 4-heptanone, 2-pentanone and 2-butanone. A comparative analysis of unfrozen and frozen-thawed urine (stored at room temperature for 0, 1 and 8 h) showed that samples retained the same number of compounds although variations in the peak areas for some compounds were observed.

MATERIALS

Product Number

Brand

Product Description

57310-U

Supelco

SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), d_f 65 μm(PDMS/DVB, needle size 24 ga, for use with manual holder

57345-U

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SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), d_f 65 μm(PDMS/DVB, for use with autosampler, needle size 23 ga

57362-U

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SPME fiber assortment kit 5, needle size 23 ga, for use with autosampler

57325-U

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SPME fiber assortment kit 4, for use with autosampler, size 24 ga

57287-U

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SPME fiber assortment kit 4, for use with autosampler, needle size 23 ga

57285-U

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SPME fiber assortment kit 1, for use with autosampler, needle size 23 ga

57286-U

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SPME fiber assortment kit 2, for use with autosampler, needle size 23 ga

57346-U

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SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), d_f 65 μm(PDMS/DVB, fused silica fiber, for use with manual holder, needle size 23 ga

57550-U

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SPME StableFlex^™ fiber assortment kit, for use with manual holder

57324-U

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SPME fiber assortment kit 4, for use with manual holder, size 24 ga

57327-U

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SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), d_f 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with autosampler

57321-U

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SPME fiber assortment kit 2, for use with autosampler, size 24 ga

57320-U

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SPME fiber assortment kit 2, for use with manual holder, needle size 24 ga

57354-U

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SPME fiber assembly, Carbowax-Polyethylene Glycol (PEG) Coating, needle size 23 ga, d_f 60 μm(PEG, for use with autosampler

57551-U

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SPME StableFlex^™ fiber assortment kit, for use with autosampler, needle size 24 ga

57293-U

Supelco

SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), d_f 65 μm(PDMS/DVB, needle size 23 ga, PDMS/DVB StableFlex, for use with autosampler

29534-U

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Carboxen^® 569, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2 in., preconditioned, pkg of 10 ea

68554

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γ-Hexalactone, analytical standard

28718-U

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Tenax^® TA / Carboxen^® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok^® End-Fittings), pkg of 10 ea

20355

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ORBO^™ 78 HBr on Carboxen^® 564 specially cleaned (20/45), 400/200 mg, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

20358

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ORBO^™ 90 Carboxen^® 564 (20/45), 160/80 mg, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

57307

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SPME fiber assortment kit 1, for use with autosampler, needle size 24 ga

57306

Supelco

SPME fiber assortment kit 1, for use with manual holder, needle size 24 ga