K4002
Krebs-Ringer Bicarbonate Buffer
With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution
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About This Item
UNSPSC Code:
12161702
NACRES:
NA.75
Recommended Products
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
pH
6.1-6.7
solubility
water: 9.5 g/L, clear
storage temp.
2-8°C
General description
Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.
Application
Krebs-Ringer Bicarbonate Buffer has been used:
- as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
- in the pre-perifusion for islet viability and functionality studies
- in pituitary bioassay
Quantity
Formulated to contain 9.5 grams of powder per liter of medium.
Reconstitution
Supplement with 1.26 g/L sodium bicarbonate.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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