P0044
Phosphatase Inhibitor Cocktail 3
DMSO solution
Synonym(s):
Phosphatase inhibitor
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Related Categories
Application
The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been tested on extract from animal tissues including human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells. It has been optimized and tested for L-isozymes of alkaline phosphatase as measured with pNPP and for serine-threonine protein phosphatases.
Biochem/physiol Actions
This mixture contains individual components with specific inhibitory properties. Cantharidin inhibits protein phosphatase 2A. (-)-p-Bromolevamisole oxalate inhibits L-isoforms of alkaline phosphatases. Calyculin A inhibits protein phosphatases 1 and 2A.
Caution
As supplied, this product is stable for four years at the recommended 2-8°C storage temperature.
Preparation Note
This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Jinglin L Xie et al.
Nature communications, 8(1), 499-499 (2017-09-13)
The capacity to coordinate environmental sensing with initiation of cellular responses underpins microbial survival and is crucial for virulence and stress responses in microbial pathogens. Here we define circuitry that enables the fungal pathogen Candida albicans to couple cell cycle
Tetsuro Ikegami et al.
PLoS pathogens, 5(2), e1000287-e1000287 (2009-02-07)
Rift Valley fever virus (RVFV) (genus Phlebovirus, family Bunyaviridae) is a negative-stranded RNA virus with a tripartite genome. RVFV is transmitted by mosquitoes and causes fever and severe hemorrhagic illness among humans, and fever and high rates of abortions in
Changyi Ji et al.
Autophagy, 15(7), 1199-1213 (2019-02-13)
A major cellular catabolic pathway in neurons is macroautophagy/autophagy, through which misfolded or aggregation-prone proteins are sequestered into autophagosomes that fuse with lysosomes, and are degraded. MAPT (microtubule-associated protein tau) is one of the protein clients of autophagy. Given that
Sunandini Chandra et al.
FEBS letters, 593(15), 1927-1943 (2019-06-12)
Connivance of cellular factors during virus-host cell membrane fusion is poorly understood. We have recently shown that cellular villin plays an important role during membrane fusion of reconstituted Sendai virosomes with hepatocytes. Here, we employed villin-null Chinese Hamster Ovary (CHO)
Zhang Xiao Ying et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 22(21), 5383-5393 (2016-11-03)
This study was performed to further our understanding of the biological and genetic basis of follicular lymphoma and to identify potential novel therapy targets. We analyzed previously generated whole exome sequencing data of 23 follicular lymphoma cases and one transformed
Articles
Information on Phosphatase Inhibitor Cocktail for researchers and students from MilliporeSigma. Phosphorylation is a reversible protein post-translational modification.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service