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95290

Sigma-Aldrich

Methyl Blue

for microscopy (Bot., Hist.), indicator (pH 9.4-14.0)

Synonym(s):

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

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About This Item

Empirical Formula (Hill Notation):
C37H27N3Na2O9S3
CAS Number:
28983-56-4
Molecular Weight:
799.80
Colour Index Number:
42780
EC Number:
249-352-9
MDL number:
MFCD00058509
UNSPSC Code:
12171500
PubChem Substance ID:
329770433
NACRES:
MA.02

grade

for microscopy (Bot., Hist.)
indicator (pH 9.4-14.0)

Quality Level

200

form

powder or crystals

pH

9.4-14

solubility

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥200 at 310-320 nm
≥400 at 595-605 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

InChI key

MCPLVIGCWWTHFH-UHFFFAOYSA-L

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General description

Methyl Blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

Application

Methyl Blue has been used:
  • to stain leaf disks for callose visualization within the cells
  • to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
  • as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
  • in Mann′s stain as it involves a methyl blue-eosin mixture

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

>572.0 °F - Pensky-Martens closed cup

Flash Point(C)

> 300 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB9353
BCBV2236
BCBR0621V
BCBM0641V
BCBH0469V

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Marie-Eve Asselin et al.
Pediatric dentistry, 30(1), 29-33 (2008-04-12)
The purpose of this in vitro study was to investigate and compare the microleakage-occurring after the placement of a light-curing sealant-to unground permanent enamel which had been previously conditioned using 3 different application protocols. Sixty-three molars were randomly distributed in
C Sahoo et al.
Journal of hazardous materials, 215-216, 302-310 (2012-03-21)
Photocatalytic degradation of methyl blue (MYB) was studied using Ag(+) doped TiO(2) under UV irradiation in a batch reactor. Catalytic dose, initial concentration of dye and pH of the reaction mixture were found to influence the degradation process most. The
Shengwei Liu et al.
Nanoscale, 4(10), 3193-3200 (2012-04-07)
Mesoporous TiO(2)-graphene nanocomposites are fabricated in high yield via two successive steps: (1) hydrothermal hydrolysis of Ti(SO(4))(2) in an acidic suspension of graphene oxide to gain TiO(2)-graphene oxide nanocomposites; (2) UV-assisted photocatalytic reduction of graphene oxide to get the TiO(2)-graphene
Baogan Peng et al.
European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 16(1), 33-38 (2006-02-24)
This article was a preliminary report of prospective clinical trial of a group of patients with chronic discogenic low back pain who met the criteria for lumbar interbody fusion surgery but were treated instead with an intradiscal injection of methylene
Lijun Dong et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 66(4-5), 1317-1322 (2007-02-06)
A novel flow injection method with resonance light scattering detection was developed for the determination of total protein concentrations. This method is based on the enhancement of RLS signals from Methyl Blue (MB) by protein. The enhanced RLS intensities at
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