ST1641

Anti-MAP4K4 Mouse mAb (4A5)

liquid, clone 4A5, Calbiochem^®

• Synonym(s): Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4A5, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAP4K4(9448)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~140 kDa MAP4K4 protein.

Recognizes the ~140 kDa MAP4K4 protein in K-562 cells and human breast cancer tissue.

This Anti-MAP4K4 Mouse mAb (4A5) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of MAP4K4.

Immunogen

A recombinant polypeptide corresponding to amino acids of 611-710 human MAP4K4, expressed as a GST fusion protein

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
K-562 cells, Human breast cancer tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable