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SHC002H

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, High Titer

Targets no known mammalian genes

Synonym(s):

MISSION®, MISSION® Control Transduction Particles

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

Quality Level

100
200

product line

MISSION®

concentration

≥1x109 VP/ml (via p24 assay)

technique(s)

capture ELISA: 109 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

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General description

This shRNA non-mammalian control was designed using our Turbo GFP sequence and may cause some knockdown of tGFP. For maximum knockdown of tGFP, please refer to SHC004, SHC004V, SHC004H, SHC204, or SHC204V.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Components

High titer controls are provided as 10 x 20 μl aliquots.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nicole T Watt et al.
Diabetes, 66(11), 2808-2821 (2017-08-24)
Shc homology 2-containing inositol 5' phosphatase-2 (SHIP2) is a lipid phosphatase that inhibits insulin signaling downstream of phosphatidylinositol 3-kinase (PI3K); its role in vascular function is poorly understood. To examine its role in endothelial cell (EC) biology, we generated mice
Min Ling et al.
Cell & bioscience, 7, 27-27 (2017-05-27)
Bone degenerative disorders like osteoporosis may be initiated by age-related shifts in anabolic and catabolic responses that control bone homeostasis. Although there are studies suggesting that metabolic changes occur with stem cell differentiation, the molecular mechanisms governing energy metabolism and
Karin M E Andersson et al.
Oncotarget, 6(24), 20043-20057 (2015-09-08)
Follicular T helper (Tfh) cells are recognized by the expression of CXCR5 and the transcriptional regulator Bcl-6. Tfh cells control B cell maturation and antibody production, and if deregulated, may lead to autoimmunity. Here, we study the role of the

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