M0404

Murashige and Skoog Basal Medium

powder, suitable for plant cell culture, with Gamborg′s vitamins

• Synonym(s): MS0 Medium, MSO Medium, Murashige and Skoog (Gamborg Modified) Basal Media, MS Basal Medium
• MDL number: MFCD00240976
• UNSPSC Code: 12352207
• NACRES: NA.72

Properties

• Quality Level: 200
• form: powder
• technique(s): cell culture | plant: suitable
• application(s): agriculture
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

Murashige and Skoog Basal Medium has been used:

• to germinate the seeds for seedling growth and germination studies
• in the suspension culture of Nicotiana plumbaginifolia for transformation
• in the root culture of hairy clone A. artemisiifolia T4

Formula variant

With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Safety Information

• Pictograms: GHS03,GHS07
• Signal Word: Warning
• Hazard Statements: H272,H319
• Precautionary Statements: P210 - P220 - P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Ox. Sol. 3
• Storage Class Code: 5.1B - Oxidizing hazardous materials
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable