- A Single-Step Enrichment Medium for Nonchromogenic Isolation of Healthy and Cold-Injured Salmonella spp. from Fresh Vegetables.
A Single-Step Enrichment Medium for Nonchromogenic Isolation of Healthy and Cold-Injured Salmonella spp. from Fresh Vegetables.
Foodborne pathogens and disease (2017-01-05)
Hong-Seok Kim, Dasom Choi, Il-Byeong Kang, Dong-Hyeon Kim, Jin-Hyeok Yim, Young-Ji Kim, Jung-Whan Chon, Deog-Hwan Oh, Kun-Ho Seo
PMID28051328
ABSTRACT
Culture-based detection of nontyphoidal Salmonella spp. in foods requires at least four working days; therefore, new detection methods that shorten the test time are needed. In this study, we developed a novel single-step Salmonella enrichment broth, SSE-1, and compared its detection capability with that of commercial single-step ONE broth-Salmonella (OBS) medium and a conventional two-step enrichment method using buffered peptone water and Rappaport-Vassiliadis soy broth (BPW-RVS). Minimally processed lettuce samples were artificially inoculated with low levels of healthy and cold-injured Salmonella Enteritidis (10
MATERIALS