Efficient hydrolytic reaction of an acetate ester with fungal lipase in a liquid-liquid interface bioreactor (L-L IBR) using CaCO₃-coated ballooned microsphere.

In a liquid-liquid interface bioreactor using a CaCO₃-coated ballooned microsphere, 2-ethylhexyl acetate was efficiently hydrolyzed to 2-ethyl-1-hexanol with Absidia coerulea NBRC 4423 compared with using talc-coated or non-coated ballooned microsphere. It was assumed that CaCO₃ brought about stabilization of lipase by Ca²⁺ and maintenance of medium pH.