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MAK056

Sigma-Aldrich

Choline/Acetylcholine Quantification Kit

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

Synonym(s):

Choline/Acetylcholine Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

usage

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine, a critical neurotransmitter in both the peripheral and central nervous systems.

Application

Choline/Acetylcholine Quantification Kit has been used to quantify the amounts of choline and acetylcholine in samples.

Suitability

Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.

Principle

In this assay, choline concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the choline present. Acetylcholine levels can be determined by adding acetylcholinesterase to the reaction, which hydrolyzes acetylcholine to choline and acetate.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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