1.52320
Chromolith® HighResolution RP-18 endcapped
L × I.D. 25 mm × 2 mm HPLC column
Synonym(s):
Chromolith® HighResolution RP-18 endcapped 25-2 HPLC column
About This Item
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Quality Level
feature
endcapped
extent of labeling
18% Carbon loading
parameter
200 bar pressure
50 °C max. temp.
technique(s)
HPLC: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
25 mm × 2 mm
matrix
highly porous
monolithic silica
matrix active group
C18 bonding phase, octadecylsilane (ODS)
pore size
1.5 μm macroporosity
15 nm mesoporosity
operating pH range
2-7.5
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General description
Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column.
The column dimension of 25 mm length and 2 mm I.D. is the best choice for very rapid separations meeting UHPLC and LC-MS needs.
Chromolith® HighResolution RP-18 endcapped columns with 2 mm internal diameter are ideal for use with UHPLC or UPLC instruments, thanks to their very small internal volumes. A particular benefit is very fast analysis, reduced sample-preparation and the low column backpressure. Ultra-high performance and extremely low operating pressure make Chromolith® 2 mm I.D. columns truly unique. Chromolith® and Chromolith® HighResolution columns are perfectly suitable for LC-MS use; however, the column dimension of 2 mm I.D. or below is preferred to be used in LC-MS methods.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-2 guard cartridges (3 pieces) [1.52325] with the Chromolith® Guard Cartridge Holder 5 mm x 2 mm [1.52355]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
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