1.11714

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide

for biochemistry

• Synonym(s): 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, Thiazolyl blue, MTT
• Empirical Formula (Hill Notation): C₁₈H₁₆BrN₅S
• CAS Number: 298-93-1
• Molecular Weight: 414.32
• MDL number: MFCD00011964
• UNSPSC Code: 12352107
• EC Index Number: 206-069-5

Properties

• Quality Level: 100
• form: solid
• mp: 195 °C
• solubility: 10 g/L
• storage temp.: 2-30°C
• InChI: 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
• InChI key: AZKSAVLVSZKNRD-UHFFFAOYSA-M

Description

Application

<ul>
<li><strong>Anal Biochem, 2017</strong>: Comparison of MTT and Neutral Red assays to assess cell viability in the presence of copper compounds, highlighting the versatility and sensitivity of the MTT assay in cytotoxicity testing (Gomez Perez et al., 2017).</li>
<li><strong>Indian J Pharmacol, 2016</strong>: Evaluation of the cytotoxic potential of various Indian fruit peels on liver cancer cells (HepG2) using MTT assay, providing insights into natural product research and potential anticancer properties (Garg et al., 2016).</li>
<li><strong>ACS Appl Mater Interfaces, 2015</strong>: Application in the real-time evaluation of live cancer cells, combining surface plasmon resonance and electrochemical methodologies with MTT assays for enhanced accuracy in viability testing (Wu et al., 2015).</li>
</ul>

Analysis Note

Assay (argentometric): ≥ 98
Identity (IR-spectrum): passes test
Spec. Absorptivity A 1%/1cm (242 nm; 0.001 %; 1 cm; methanol): 480 - 530
Heavy metals (as Pb): ≤ 0.001 %
Sulfated ash: ≤ 0.5 %

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Warning
• Hazard Statements: H315 - H319 - H335 - H341
• Precautionary Statements: P202 - P261 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable