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Key Documents

07-820

Sigma-Aldrich

Anti-phospho-EGFR (Tyr845) Antibody

Upstate®, from rabbit

Synonym(s):

Anti-ERBB, Anti-ERBB1, Anti-ERRP, Anti-HER1, Anti-NISBD2, Anti-PIG61, Anti-mENA

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1

UniProt accession no.

P00533

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr845)

Gene Information

human ... EGFR(1956)

Specificity

Predicted to cross-react with mouse and rat based on sequence homology (100%), but have not been tested.
Recognizes phospho-EGFR (Tyr845).

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr845)

Application

Immunoblot Analysis: A 1:1000 dilution of this lot detected phospho-EGFR (Tyr845) in RIPA lysates from EGF stimulated A431 cells.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
This Anti-phospho-EGFR (Tyr845) Antibody is validated for use in IC, IF, IH, WB for the detection of phospho-EGFR (Tyr845).

Quality

Routinely evaluated by immunoblot.

Target description

185 kDa

Physical form

Affinity Purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
ImmunoAffinity Purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
EGF stimulated A431 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Essential role for epidermal growth factor receptor in glutamate receptor signaling to NF-kappaB.
Sitcheran, R; Comb, WC; Cogswell, PC; Baldwin, AS
Molecular and cellular biology null
G R Johnson et al.
The Journal of biological chemistry, 268(4), 2924-2931 (1993-02-05)
The COOH-terminal half of the amphiregulin (AR) molecule has sequence homology to epidermal growth factor (EGF). The ability of AR to elicit in vivo phosphorylation of the EGF receptor (EGFR) and p185erbB2 was studied in four human epithelial cell lines
Cytotoxic properties of DAB486EGF and DAB389EGF, epidermal growth factor (EGF) receptor-targeted fusion toxins.
Shaw, J P, et al.
The Journal of Biological Chemistry, 266, 21118-21124 (1991)
Polymorphonuclear leukocytes-mediated lysis of A431 cells induced by IgG1 mouse anti-epidermal growth factor receptor monoclonal antibodies.
Kawamoto, T, et al.
In Vitro Cellular & Developmental Biology, 28A, 782-786 (1992)
Changes in retinal cell fate induced by overexpression of EGF receptor
Lillien, L.
Nature, 377, 158-162 (1995)

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