mRNA Research

Synthetic mRNA is viewed as a viable and functional replacement for mature mRNA in both in vitro and in vivo research projects, including therapeutic development and delivery. Our comprehensive offering of premier tools and reagents helps you realize the potential of synthetic mRNA in your biopharmaceutical and life science research applications.

We provide a diverse portfolio of premium products to aid in your research, from tools and kits to isolate and purify total RNA to cells and enzymes that accelerate the synthesis of mRNA.

Antigen Discovery Products

mRNA has demonstrated significant potential for the development of therapeutic approaches in infectious disease, genetic conditions, heart disease, oncology, and more. We support mRNA antigen discovery with:

• GenElute™ Viral RNA Purification and GenElute-E Viral RNA/DNA kits, which rapidly isolate and purify total RNA from viral sources
• Easy-to-understand RT qPCR Reagents for viral detection, combining the power of reverse transcriptase with taq-directed antibody into convenient ReadyMixes for both SYBR® Green-based and probe-based applications
• SeqPlex™-1 WGA & WTA Kits for Illumina® NGS, for amplification of total RNA for mRNA
• A complete portfolio of cell culture tools for viral propagation, offering a wide range of tools for manufacturing monoclonal antibodies, therapeutic proteins, and vaccines
• A comprehensive selection of enzymes and reagents used in mass spectrometry analysis, including isotope-labeled protein and antibody standards, proteases, and tagging solutions
• Flexible and fully customizable CRISPR and RNAi screens and tools to facilitate genome wide and targeted antigen discovery

mRNA Synthesis

For in vitro delivery of mRNA for applications such as stem cell induction, protein replacement, cancer immunotherapies, or vaccine modalities, synthetic mRNA has become a critical tool in diverse biopharmaceutical approaches. Our portfolio of mRNA synthesis products includes:

• Molecular cloning tools for plasmid DNA, which researchers can use to modify or create a gene of interest and produce synthetic mRNA through cell-based or cell-free systems
• A complete set of PCR tools to fulfill your PCR amplification workflow and create synthetic mRNA through dependable PCR reagents and kits, whole genome amplification consumables and kits, and nucleic acid templates
• The GenElute™ PCR Clean-up kit, which rapidly purifies single- or double-stranded PCR products or linearized pDNA for use in synthesis of mRNA
• A wide variety of in vitro transcription enzymes and reagents, including recombinant and bacteriophage RNA polymerase and in vitro purification enzymes and buffers including precipitation and resuspension reagents, plus cellulose fibers for the removal of dsRNA
• GenElute™ Direct mRNA Miniprep kit for smaller-scale purification. At larger scales,tangential flow filtration (TFF) can be achieved using Pellicon® Cassettes

Lipid Nanoparticle (LNP) Selection and Formulation

Lipid nanoparticles (LNPs) are critical vehicles for mRNA delivery - encapsulating and conveying mRNA safely to cells. LNP selection is integral at the earliest stages of vaccine and therapeutic mRNA delivery research. We offer a comprehensive portfolio for lipid selection and LNP formulation, including:

• Non-GMP lipids for use in mRNA targeting and delivery research, such as cationic lipids, PEG and structural lipids, and cholesterol
• NanoFabTx™ reagent and microfluidic device kits provide a ready-to-use platform to screen multiple formulations, and ultimately synthesize mRNA or drug-encapsulated liposomes for preclinical assessments.