TLC Analysis of Nucleosides on HPTLC Silica gel 60 RP-18 WF254s

plate

HPTLC pre-coated plate RP-18 WF 254s with concentrating zone (1.13124.0001)

eluent

[A] water; [B] ethanol; (95:5, A:B)

migration distance

7 cm

chamber

normal chamber without chamber saturation

sample

5 μL (0.005%)

detector

in situ evaluation with TLC/HPTLC scanner (Camag) UV 265 nm