HPLC Analysis of Bromelain on TSKgel^® BioAssist^® S vs. Competitive S Column

column

TSKgel Bio Assist S, 5 cm x 4.6 mm I.D., 7 μm particles (819686)

column

Competitive S-type column, 5 cm x 5 mm I.D.

mobile phase

[A] 20 mM sodium phophate buffer, pH 7.0; [B] 20 mM sodium phophate buffer, 0.5 M NaCl, pH 7.0

gradient

0 to 100% B in 20 min (0 to 100% B in 30 min for competitive column)

flow rate

0.8 mL/min (1.0 mL/min for competitive column)

column temp.

25 °C

detector

UV, 280 nm

sample

crude bromelain, 0.01 μg/mL