HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 0-16 Min. Window
CONDITIONS
column
Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)
mobile phase
[A] (95:5), 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (50:50), 0.1%TFA in water:0.1% TFA in acetonitrile
gradient
0-100% B in 65 min
flow rate
1 mL/min
column temp.
30 °C
detector
UV, 215 nm
injection
50μL carboxymethylated apohemoglobin tryptic digest in 50 mM ammonium bicarbonate
Description
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany