Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

267007

Sigma-Aldrich

Nickel, foil, thickness 0.25 mm, 99.995% trace metals basis

267058

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.99% trace metals basis

268283

Sigma-Aldrich

Nickel, powder, <1 μm, 99.8% trace metals basis

357634

Sigma-Aldrich

Nickel, wire, diam. 0.25 mm, ≥99.9%

268259

Sigma-Aldrich

Nickel, foil, thickness 0.125 mm, ≥99.9%

GF11244259

Nickel, rod, 200mm, diameter 25.4mm, 99.98%

GF45193697

Nickel, rod, 100mm, diameter 2.0mm, annealed, 99%

GF61468421

Nickel, foil, 10mm disks, thickness 0.125mm, as rolled, 99.99+%

GF20399159

Nickel, tube, 100mm, outside diameter 1.5mm, inside diameter 1.4mm, wall thickness 0.05mm, hard, 99.5%

GF85665842

Nickel, foil, 15mm disks, thickness 0.005mm, as rolled, 99.9%

GF08912831

Nickel, foil, 10mm disks, thickness 0.15mm, annealed, 99%

GF01381407

Nickel, tube, 100mm, outside diameter 1.8mm, inside diameter 1.6mm, wall thickness 0.1mm, hard, 99.5%

GF21754100

Nickel, rod, 500mm, diameter 12.7mm, 99.98%

GF34230800

Nickel, rod, 500mm, diameter 3.0mm, 99%

GF97607829

Nickel, foil, 10mm disks, thickness 0.50mm, annealed, 99%

GF51944665

Nickel, rod, 200mm, diameter 3.0mm, 99%

GF65882874

Nickel, foil, 15mm disks, thickness 0.0075mm, 99.9%

GF39205931

Nickel, rod, 100mm, diameter 25.4mm, 99.98%

GF51615996

Nickel, foil, 10mm disks, thickness 0.5mm, annealed, 99.98%

GF82992145

Nickel, foil, 10mm disks, thickness 0.05mm, as rolled, 99.98%

GF87663303

Nickel, tube, 1000mm, outside diameter 2.5mm, inside diameter 2mm, wall thickness 0.25mm, hard, 99.5%

GF91609555

Nickel, rod, 200mm, diameter 3.3mm, 99.99+%

GF72210888

Nickel, foil, 15mm disks, thickness 0.0075mm, 99.95%

GF57511380

Nickel, tube, 100mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, hard, 99.5%

GF96686140

Nickel, tube, 1000mm, outside diameter 7.11mm, inside diameter 6.57mm, wall thickness 0.27mm, hard, 99.5%

GF50091320

Nickel, tube, 1000mm, outside diameter 2.0mm, inside diameter 1.6mm, wall thickness 0.2mm, hard, 99.5%

GF16014844

Nickel, rod, 500mm, diameter 2.0mm, annealed, 99%

GF69613825

Nickel, rod, 200mm, diameter 12.0mm, 99%

GF62384328

Nickel, tube, 100mm, outside diameter 0.8mm, inside diameter 0.64mm, wall thickness 0.08mm, as drawn, 99.5%

GF83887723

Nickel, tube, 1000mm, outside diameter 2.8mm, inside diameter 1.8mm, wall thickness 0.5mm, hard, 99.5%