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Growth and Purification of Vaccinia Virus Stocks for MPM Imaging.

Methods in molecular biology (Clifton, N.J.) (2019-06-27)
Glennys V Reynoso, John P Shannon, Jeffrey L Americo, James Gibbs, Heather D Hickman
ABSTRACT

This chapter provides methods for the propagation, purification, and titration of vaccinia virus (VACV) and the highly attenuated strain-modified vaccinia Ankara (MVA). Additionally, we provide information on VACV recombinants we have used for intravital imaging with multiphoton excitation.

MATERIALS
Product Number
Brand
Product Description

Roche
Bovine Serum Albumin Fraction V, protease-free, from bovine serum