D6665

2′,7′-Dichlorofluorescein

~90% purity (TLC) (85- 100% purity), powder

• Synonym(s): Dichlorofluorescein; Fluorescein 27
• Empirical Formula (Hill Notation): C₂₀H₁₀Cl₂O₅
• CAS Number: 76-54-0
• Molecular Weight: 401.20
• Beilstein: 58009
• EC Number: 200-968-6
• MDL number: MFCD00005047
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894041
• NACRES: NA.47

Properties

• product name: 2′,7′-Dichlorofluorescein, suitable for use as an indicator in chloride titration, ~90% (TLC), powder
• Assay: ~90% (TLC)
• form: powder
• mp: 280 °C (dec.) (lit.)
• solubility: ethanol: 25 mg/mL
• density: 0.79 g/cm³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
• InChI: 1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
• InChI key: VFNKZQNIXUFLBC-UHFFFAOYSA-N

Description

General description

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).citation

Application

2′,7′-Dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves