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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS0
Reactivity
Rat CD86
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Description
The hybridoma cell line OX-48 secretes monoclonal IgG antibodies reactive against rat CD86.
Immunogen
Activated rat T cells
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
Subculture Routine
Seed at 5 x 104 cells/ml (5 x 105 cells/ml at resuscitation). Subculture when cells reach 5 x 105 -1 x 106 cells/ml (usually after 3-4 days), 5% CO2, 37°C. 20% FBS can be used at resuscitation.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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