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C8862

SAFC

EX-CELL® CHO DHFR− Medium

Animal-component free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

phenol red: no
glucose: yes
NaHCO3: yes
HEPES: yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

Related Categories

Application

This animal component-free medium (containing no hypoxanthine or thymidine) is formulated to maximize cell growth and recombinant protein production using the Dihydrofolate Reductase (DHFR) gene amplification system in DHFR- Chinese Hamster Ovary (CHO) cells.

Features and Benefits

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. This medium supports and maintains high cell densities for extended periods, resulting in increased productivity.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Selection Process Development in a GS Knock-Out CHO Host Cell Line: The Effects of MSX Addition on Clones Generated in an MSX-Free Process

The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992).

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Cell lines for Recombinant Protein Expression

Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines at sigmaaldrich.com

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