N6019
Neutralizing Buffer
for Northern and Southern blotting, powder
Synonym(s):
Neutralizing Buffer for Blotting Techniques
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About This Item
UNSPSC Code:
12161700
NACRES:
NA.25
Recommended Products
grade
for molecular biology
Quality Level
form
powder
technique(s)
Southern blotting: suitable
foreign activity
DNase, RNase, none detected
storage temp.
room temp
General description
Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.
Application
Suitable for neutralizing the gel before transfer to membranes during Southern procedures.
Components
The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
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